Journal: The Journal of Clinical Investigation

Article Title: Acute depletion of activated memory B cells involves the PD-1 pathway in rapidly progressing SIV-infected macaques

doi: 10.1172/JCI43271

Figure Lengend Snippet: (A) Representative dot plots showing percentages of annexin V+ mBAct cells following culture with Huh-7.5 or Huh-7.5-PD-L1 cells. Summary of annexin V binding on (B) mBAct cells and (C) naive B cells following culture with Huh-7.5 or Huh-7.5-PD-L1 cells. Symbols represent individual SIV+ animals (n = 16); data obtained from 4 independent experiments. (D) In vitro blockade of PD-1 enhanced total IgM-, IgG-, and SIV-specific ASCs following polyclonal stimulation of PBMCs from SIV-infected RMs. Each data point represents an individual animal, and horizontal lines represent medians. **P < 0.01.

Article Snippet: On day 5 of culture, 96-well ELISpot plates (Millipore) were coated overnight at 4°C with affinity-purified goat anti-monkey IgM (10 μg/ml; Rockland Immunochemicals), IgG (10 μg/ml; Rockland Immunochemicals), or SIV mac239 gp130 (1 μg/ml; obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH).

Techniques: Binding Assay, In Vitro, Infection